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Vet Microbiol. 2014 Jul 16;171(3-4):422-31. doi: 10.1016/j.vetmic.2014.02.014. Epub 2014 Feb 16.

Antimicrobial-resistant Enterobacteriaceae from humans and wildlife in Dzanga-Sangha Protected Area, Central African Republic.

Author information

1
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, 61242 Brno, Czech Republic; Institute of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, 61242 Brno, Czech Republic.
2
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, 61242 Brno, Czech Republic. Electronic address: albrechtovakaterina@gmail.com.
3
Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, v.v.i., Kvetna 8, 60365 Brno, Czech Republic; Liberec Zoo, Masarykova 1347/31, 46001 Liberec, Czech Republic; Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branisovska 31, 37005 Ceske Budejovice, Czech Republic; Department of Pathology and Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, 61242 Brno, Czech Republic.
4
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, 61242 Brno, Czech Republic; CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, 61242 Brno, Czech Republic.
5
Institute of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, 61242 Brno, Czech Republic; CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, 61242 Brno, Czech Republic.
6
WWF, Dzanga Sangha Protected Areas, Bangui, Central African Republic.
7
Department of Anthropology, Durham University, Dawson Building, South Road, Durham, DH1 3LE, United Kingdom.
8
Department of Pathology and Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, 61242 Brno, Czech Republic.
9
Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branisovska 31, 37005 Ceske Budejovice, Czech Republic; Department of Pathology and Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, 61242 Brno, Czech Republic; CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, 61242 Brno, Czech Republic.

Abstract

Antimicrobial resistance is a worldwide concern of public health. Unfortunately, resistant bacteria are spreading to all ecosystems, including the strictly protected ones. We investigated antimicrobial resistance in gastrointestinal Enterobacteriaceae of wild mammals and people living within Dzangha-Sangha Protected Areas, Central African Republic, with an emphasis on extended-spectrum β-lactamase (ESBL) and plasmid-mediated quinolone resistance (PMQR) genes. We compare resistance genes found in microbiota of humans, gorillas habituated and unhabituated to humans and other wildlife. In gorillas, we additionally investigate the presence of ESBL resistant isolates after treatment by ceftiofur. We found a considerable prevalence of multiresistant Enterobacteriaceae isolates with ESBL and PMQR genes in humans (10% and 31%, respectively). Among wildlife the most significant findings were CTX-M-15-producing Klebsiella pneumoniae in a habituated gorilla and a multiresistant Escherichia coli isolate with gene qepA in an unhabituated gorilla. Other isolates from wildlife were mostly represented by qnrB-harboring Citrobacter spp. The relatedness of resistant E. coli was investigated in a PFGE-based dendrogram; isolates from gorillas showed less than 80% similarity to each other and less than 80% similarity to human isolates. No ESBL-producing isolates were found in animals treated by ceftiofur. Although we did not detect any bacterial clone common to wildlife and humans, we detected an intersection in the spectrum of resistance genes found in humans and gorillas, represented by blaCTX-M-15 and qepA.

KEYWORDS:

Africa; ESBL; Enterobacteriaceae; Gorilla; PMQR; Resistance

PMID:
24636162
DOI:
10.1016/j.vetmic.2014.02.014
[Indexed for MEDLINE]

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