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Nat Cell Biol. 2014 Apr;16(4):322-34. doi: 10.1038/ncb2921. Epub 2014 Mar 16.

Septins promote F-actin ring formation by crosslinking actin filaments into curved bundles.

Author information

1
Institut de Biologie du Développement de Marseille, CNRS UMR 7288, Aix-Marseille Université, 13288 Marseille, France.
2
1] FOM Institute AMOLF, 1098 XG Amsterdam, The Netherlands [2].
3
1] Institut Curie, CNRS UMR 168, 75231 Paris, France [2].
4
Institut Fresnel, CNRS UMR 7249, Aix-Marseille Université, Ecole Centrale Marseille, 13397 Marseille, France.
5
FOM Institute AMOLF, 1098 XG Amsterdam, The Netherlands.

Abstract

Animal cell cytokinesis requires a contractile ring of crosslinked actin filaments and myosin motors. How contractile rings form and are stabilized in dividing cells remains unclear. We address this problem by focusing on septins, highly conserved proteins in eukaryotes whose precise contribution to cytokinesis remains elusive. We use the cleavage of the Drosophila melanogaster embryo as a model system, where contractile actin rings drive constriction of invaginating membranes to produce an epithelium in a manner akin to cell division. In vivo functional studies show that septins are required for generating curved and tightly packed actin filament networks. In vitro reconstitution assays show that septins alone bundle actin filaments into rings, accounting for the defects in actin ring formation in septin mutants. The bundling and bending activities are conserved for human septins, and highlight unique functions of septins in the organization of contractile actomyosin rings.

Comment in

PMID:
24633326
DOI:
10.1038/ncb2921
[Indexed for MEDLINE]

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