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Methods. 2014 Jun 15;68(1):105-15. doi: 10.1016/j.ymeth.2014.02.034. Epub 2014 Mar 12.

Methods for studying metabolism in Drosophila.

Author information

1
Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112-5330, USA.
2
Department of Biochemistry and the Metabolomics Core Research Facility, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.
3
Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112-5330, USA. Electronic address: carl.thummel@genetics.utah.edu.

Abstract

Recent research using Drosophila melanogaster has seen a resurgence in studies of metabolism and physiology. This review focuses on major methods used to conduct this work. These include protocols for dietary interventions, measurements of triglycerides, cholesterol, glucose, trehalose, and glycogen, stains for lipid detection, and the use of gas chromatography-mass spectrometry (GC-MS) to detect major polar metabolites. It is our hope that this will provide a useful framework for both new and current researchers in the field.

KEYWORDS:

Drosophila; Metabolism; Metabolomics; Physiology

PMID:
24631891
PMCID:
PMC4048761
DOI:
10.1016/j.ymeth.2014.02.034
[Indexed for MEDLINE]
Free PMC Article

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