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Water Res. 2014 May 15;55:304-12. doi: 10.1016/j.watres.2014.02.011. Epub 2014 Feb 14.

Microbial communities from different types of natural wastewater treatment systems: vertical and horizontal flow constructed wetlands and biofilters.

Author information

1
Health and Environmental Microbiology Laboratory, Optics and Optometry Department & AQUASOST - UNESCO Chair on Sustainability, Universitat Politècnica de Catalunya, Edifici Gaia, Pg. Ernest Lluch/Rambla Sant Nebridi, Terrassa 08222, Spain. Electronic address: jordi.morato@upc.edu.
2
Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
3
Aarhus University, Department of Bioscience, Ole Worms Allé 1, Building 1135, 8000 Århus C, Denmark.
4
Facultad de Ciencias Biológicas y Ambientales, Universidad de León, Campus de Vegazana s/n, 24071 León, Spain.
5
Health and Environmental Microbiology Laboratory, Optics and Optometry Department & AQUASOST - UNESCO Chair on Sustainability, Universitat Politècnica de Catalunya, Edifici Gaia, Pg. Ernest Lluch/Rambla Sant Nebridi, Terrassa 08222, Spain.

Abstract

The prokaryotic microbial communities (Bacteria and Archaea) of three different systems operating in Denmark for the treatment of domestic wastewater (horizontal flow constructed wetlands (HFCW), vertical flow constructed wetlands (VFCW) and biofilters (BF)) was analysed using endpoint PCR followed by Denaturing Gradient Gel Electrophoresis (DGGE). Further sequencing of the most representative bacterial bands revealed that diverse and distinct bacterial communities were found in each system unit, being γ-Proteobacteria and Bacteroidetes present mainly in all of them, while Firmicutes was observed in HFCW and BF. Members of the Actinobacteria group, although found in HFCW and VFCW, seemed to be more abundant in BF units. Finally, some representatives of α, β and δ-Proteobacteria, Acidobacteria and Chloroflexi were also retrieved from some samples. On the other hand, a lower archaeal diversity was found in comparison with the bacterial population. Cluster analysis of the DGGE bacterial band patterns showed that community structure was related to the design of the treatment system and the organic matter load, while no clear relation was established between the microbial assemblage and the wastewater influent.

KEYWORDS:

Biofilters; Constructed wetlands; Domestic wastewater; Microbial community; PCR-DGGE

PMID:
24631879
DOI:
10.1016/j.watres.2014.02.011
[Indexed for MEDLINE]

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