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J Immunol Methods. 2014 Apr;406:43-50. doi: 10.1016/j.jim.2014.03.001. Epub 2014 Mar 13.

IgE to penicillins with different specificities can be identified by a multiepitope macromolecule: Bihaptenic penicillin structures and IgE specificities.

Author information

1
Research Laboratory, IBIMA, Regional University Hospital of Malaga, UMA, Plz. del Hospital Civil s/n, 29009 Malaga, Spain. Electronic address: adriana.ariza@fundacionimabis.org.
2
Allergy Unit, IBIMA, Regional University Hospital of Malaga, UMA, Plza. Hospital Civil s/n, 29009 Málaga, Spain. Electronic address: esther.barrionuevo@hotmail.com.
3
Research Laboratory, IBIMA, Regional University Hospital of Malaga, UMA, Plz. del Hospital Civil s/n, 29009 Malaga, Spain. Electronic address: mayorga.lina@gmail.com.
4
Research Laboratory, IBIMA, Regional University Hospital of Malaga, UMA, Plz. del Hospital Civil s/n, 29009 Malaga, Spain; Andalusian Centre for Nanomedicine and Biotechnology - BIONAND, C/Severo Ochoa 35, 29590, Campanillas, Malaga, Spain. Electronic address: maribel.montanez@fundacionimabis.org.
5
Andalusian Centre for Nanomedicine and Biotechnology - BIONAND, C/Severo Ochoa 35, 29590, Campanillas, Malaga, Spain; Department of Organic Chemistry, Faculty of Sciences, University of Malaga, Campus de Teatinos s/n, 29071 Malaga, Spain. Electronic address: inestrosa@uma.es.
6
Andalusian Centre for Nanomedicine and Biotechnology - BIONAND, C/Severo Ochoa 35, 29590, Campanillas, Malaga, Spain; Department of Organic Chemistry, Faculty of Sciences, University of Malaga, Campus de Teatinos s/n, 29071 Malaga, Spain. Electronic address: ajruizs@uma.es.
7
Laboratory of Cellular and Molecular Pathology in Nutrition, Faculty of Medicine, University of Nancy, 9 avenue de la Forêt de Haye, B.P. 184, 54505 Vandoeuvre-les-Nancy Cedex, France. Electronic address: rosa-maria.gueant-rodriguez@univ-lorraine.fr.
8
Research Laboratory, IBIMA, Regional University Hospital of Malaga, UMA, Plz. del Hospital Civil s/n, 29009 Malaga, Spain. Electronic address: tahiadfd@gmail.com.
9
Laboratory of Cellular and Molecular Pathology in Nutrition, Faculty of Medicine, University of Nancy, 9 avenue de la Forêt de Haye, B.P. 184, 54505 Vandoeuvre-les-Nancy Cedex, France. Electronic address: jean-louis.gueant@univ-lorraine.fr.
10
Allergy Unit, IBIMA, Regional University Hospital of Malaga, UMA, Plza. Hospital Civil s/n, 29009 Málaga, Spain. Electronic address: mjtorresj@gmail.com.
11
Laboratory of Cellular and Molecular Pathology in Nutrition, Faculty of Medicine, University of Nancy, 9 avenue de la Forêt de Haye, B.P. 184, 54505 Vandoeuvre-les-Nancy Cedex, France; Allergy Unit, IBIMA, Regional University Hospital of Malaga, UMA, Plza. Hospital Civil s/n, 29009 Málaga, Spain. Electronic address: mblancago@gmail.com.

Abstract

Quantitation of specific IgE by immunoassay is a recommended in vitro test for the diagnosis of immediate hypersensitivity reactions to betalactams (BLs), particularly when skin test results are negative. IgE antibodies that recognize the common nuclear structure of all BLs or the specific side chain structure can be mainly distinguished by immunoassays. The aim of this study was to develop an immunoassay system to detect IgE antibodies with different specificities. Cellulose discs conjugated with benzylpenicillin (BP), amoxicillin (AX) or both drugs, with poly-l-lysine (PLL) as carrier molecule, were used as solid phases in the radioallergosorbent test (RAST). Direct and inhibition radioimmunoassay studies were made to verify the structures recognized by serum IgE antibodies from penicillin-allergic patients. Our results indicated that the addition of both haptens did not decrease the capacity to capture IgE when serum specific to either BP or AX was used, at least in terms of sensitivity. In addition, the inclusion of two haptens improved significantly the levels of IgE detection in patients who recognized both BP and AX. Therefore, the use of a solid phase with a carrier molecule conjugated with two determinants (AX and BP) is helpful to recognize IgE antibodies against either of these determinants and is useful for screening sera with different specificities.

KEYWORDS:

IgE; Immediate reaction; Penicillin determinant; Radioimmunoassay; Solid phase

PMID:
24631718
DOI:
10.1016/j.jim.2014.03.001
[Indexed for MEDLINE]
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