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J Immunol Methods. 2014 Apr;406:51-7. doi: 10.1016/j.jim.2014.03.003. Epub 2014 Mar 12.

Development of hydrogel biochip for in vitro allergy diagnostics.

Author information

1
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences (EIMB RAS), Moscow, Russia; Moscow Institute of Physics and Technology (State University), Moscow, Russia.
2
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences (EIMB RAS), Moscow, Russia.
3
Federal State Budgetary Institution Polyclinic №1 of the Business Administration for the President of the Russian Federation, Moscow, Russia.
4
Dr. Fooke Laboratorien GmbH, Neuss, Germany.
5
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences (EIMB RAS), Moscow, Russia. Electronic address: arubina@biochip.ru.

Abstract

A hydrogel biochip was developed for the simultaneous quantitative determination of sIgE for 21 allergens and total IgE in human serum. The biochips are manufactured by photoinduced copolymerization of different molecules (allergens and antibodies) with gel-forming monomers resulting in the formation of three-dimensional hydrogel elements (1nl gel drops). After incubation of the biochip with the serum, the results are visualized using fluorescently labeled anti-IgE antibodies. Using biochips, serum samples from allergic patients and healthy donors were analyzed and good correlation with the results obtained using commercial EIA test systems of generally recognized quality (Dr. Fooke Laboratorien GmbH, Germany) was observed.

KEYWORDS:

Allergy diagnostics; Biochip; IgE in vitro test; Protein microarray

PMID:
24631646
DOI:
10.1016/j.jim.2014.03.003
[Indexed for MEDLINE]

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