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Int J Biol Macromol. 2014 Jun;67:112-23. doi: 10.1016/j.ijbiomac.2014.03.004. Epub 2014 Mar 12.

Structural characterization and antioxidant activities of polysaccharides from Citrus aurantium L.

Author information

1
Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin 150040, China.
2
Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin 150040, China. Electronic address: hxkuang@hotmail.com.

Abstract

Three polysaccharide fractions were obtained from Citrus aurantium L. (CAL) by sequential extraction with cold water, hot water, and 1.0M NaOH, respectively. The fractions were denoted CALA, CALB, and CALC. Structural characterization was conducted by physicochemical property, FTIR, and SEM analyses. Antioxidant activities in vivo and in vitro were also evaluated. CALB, which showed the highest activity, was further isolated to afford four purified polysaccharides (CALB-1-4) by various ion exchange and gel-filtration chromatography. Meanwhile, the purified polysaccharides were subjected to composition analysis and screened by antioxidant activity in vitro. Among the four purified polysaccharides, CALB-3 had the highest antioxidant activity and its structure was analyzed by FTIR, SEM and AFM microscopy. Overall, these results indicated that polysaccharides from CAL had potential therapeutic applications in the medical and food industries because of their antioxidant activities.

KEYWORDS:

Antioxidant activity; Citrus aurantium L.; Polysaccharide; Structural characterization

PMID:
24631547
DOI:
10.1016/j.ijbiomac.2014.03.004
[Indexed for MEDLINE]

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