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Int Immunopharmacol. 2014 May;20(1):188-96. doi: 10.1016/j.intimp.2014.02.031. Epub 2014 Mar 12.

Sour cherry (Prunus cerasus) seed extract increases heme oxygenase-1 expression and decreases proinflammatory signaling in peripheral blood human leukocytes from rheumatoid arthritis patients.

Author information

1
Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Kuwait University, Kuwait. Electronic address: fadia@hsc.edu.kw.
2
Debrecen University, Faculty of Pharmacy, Hungary.
3
Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Kuwait University, Kuwait.
4
Department of Medicine, Faculty of Medicine, Kuwait University, Kuwait.

Abstract

Sour cherry seed extract (SCE) was evaluated for its capacity to inhibit lipopolysaccharide-treated human peripheral blood T cells expressing tumor necrosis factor-alpha, and the chemokine interleukin-8. Both proteins are diagnostic biomarkers for inflammatory pathologies. Peripheral blood leukocytes from 11 rheumatoid arthritis (RA) patients and 8 healthy control subjects were co-cultured for 24h in lipopolysaccharide and the extract, then evaluated by flow cytometry for T cell activation and by enzyme-linked immunoassay for lymphocyte-associated heme oxygenase-1 (HO-1) expression. There was a dose-dependent decrease in expression of the immunophenotypes: CD3+TNF-α+, and CD3+IL8+ in cultures from RA patients to a greater extent than in cells from healthy participants. These results suggest that the extract may have a modulatory roll in RA and other inflammatory disorders via the induction of HO-1, thus abating oxidative stress and strengthening regulation of pro-inflammatory signaling pathways.

KEYWORDS:

HO-1; IL-8; Peripheral blood; Rheumatoid arthritis; Sour cherry kernel; TNF-α

PMID:
24631368
DOI:
10.1016/j.intimp.2014.02.031
[Indexed for MEDLINE]

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