Format

Send to

Choose Destination
Wilderness Environ Med. 2014 Jun;25(2):160-5. doi: 10.1016/j.wem.2014.01.006. Epub 2014 Mar 13.

Poly-L-arginine topical lotion tested in a mouse model for frostbite injury.

Author information

1
Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, CA.
2
Department of Pathology and Dermatology, Stanford University School of Medicine, Stanford, CA.
3
Division of Immunology and Rheumatology, Department of Medicine, Stanford University School of Medicine, Stanford, CA.
4
Division of Emergency Medicine, Department of Surgery, Stanford University School of Medicine, Stanford, CA. Electronic address: auerbach@stanford.edu.

Abstract

BACKGROUND:

Frostbite injury occurs when exposure to cold results in frozen tissue. We recently reported a novel mouse model for frostbite injury to be used in screening potentially therapeutic drugs and other modalities.

OBJECTIVE:

We used the mouse skin frostbite model to evaluate the effect of poly-l-arginine contained in lotion (PAL) applied topically to involved skin.

METHODS:

Sixty mice were studied in a randomized, double-blind method. Standardized 2.9-cm-diameter circles were tattooed on the mouse dorsum. Magnets snap frozen in dry ice (-78.5°C) were used to create a frostbite injury on skin within the circle as a continuous 5-minute freeze. Mice were treated with prefreeze placebo, postthaw placebo, combined prefreeze and postthaw placebo, prefreeze with PAL, postthaw with PAL, or combined prefreeze and postthaw with PAL. Appearance, healing rate, tissue loss, and histology were recorded until the wounds were healed.

RESULTS:

Application of PAL before inducing frostbite injury resulted in decreased tissue loss as compared with other treatment conditions.

CONCLUSIONS:

Applying PAL topically to frostbitten mouse skin caused decreased tissue loss. Poly-l-arginine should be studied further to determine whether it is a beneficial therapeutic modality for frostbite injury.

KEYWORDS:

cold injury; frostbite; mouse model; poly-l-arginine; wound healing

PMID:
24631228
DOI:
10.1016/j.wem.2014.01.006
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center