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Mol Cell. 2014 Apr 10;54(1):43-55. doi: 10.1016/j.molcel.2014.02.021. Epub 2014 Mar 12.

Direct regulation of the NADPH oxidase RBOHD by the PRR-associated kinase BIK1 during plant immunity.

Author information

1
The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK; RIKEN Center for Sustainable Resource Science, Plant Immunity Research Group, Suehiro-cho 1-7-22 Tsurumi-ku, Yokohama 230-0045, Japan.
2
The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
3
RIKEN Center for Sustainable Resource Science, Plant Immunity Research Group, Suehiro-cho 1-7-22 Tsurumi-ku, Yokohama 230-0045, Japan.
4
The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: cyril.zipfel@tsl.ac.uk.

Abstract

The rapid production of reactive oxygen species (ROS) burst is a conserved signaling output in immunity across kingdoms. In plants, perception of pathogen-associated molecular patterns (PAMPs) by surface-localized pattern recognition receptors (PRRs) activates the NADPH oxidase RBOHD by hitherto unknown mechanisms. Here, we show that RBOHD exists in complex with the receptor kinases EFR and FLS2, which are the PRRs for bacterial EF-Tu and flagellin, respectively. The plasma-membrane-associated kinase BIK1, which is a direct substrate of the PRR complex, directly interacts with and phosphorylates RBOHD upon PAMP perception. BIK1 phosphorylates different residues than calcium-dependent protein kinases, and both PAMP-induced BIK1 activation and BIK1-mediated phosphorylation of RBOHD are calcium independent. Importantly, phosphorylation of these residues is critical for the PAMP-induced ROS burst and antibacterial immunity. Our study reveals a rapid regulatory mechanism of a plant RBOH, which occurs in parallel with and is essential for its paradigmatic calcium-based regulation.

PMID:
24630626
DOI:
10.1016/j.molcel.2014.02.021
[Indexed for MEDLINE]
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