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Oncol Rep. 2014 May;31(5):2055-62. doi: 10.3892/or.2014.3075. Epub 2014 Mar 11.

MicroRNA-100 regulates SW620 colorectal cancer cell proliferation and invasion by targeting RAP1B.

Author information

1
Department of Colorectal and Anal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, P.R. China.
2
Department of Pediatrics, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, P.R. China.
3
Guangdong Gastrointestinal Institute, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, P.R. China.
4
Department of Gastrointestinal Anal Surgery and Institute of Gastroenterology, The Third Affiliated Hospital of Nanchang University, Nanchang 330008, P.R. China.

Abstract

MicroRNAs (miRNAs) have been demonstrated to play important roles in tumorigenesis of human cancer. Fewer studies have explored the roles of miR-100 on human colorectal cancer cell proliferation and invasion. In this study, we utilized real-time PCR to verify whether miR-100 was downregulated in human colorectal cancer tissues compared with matched adjacent normal tissues. Functional studies demonstrated that ectopic expression of miR-100 inhabits cell growth and invasion and induce apoptosis, whereas knockdown of miR-100 yielded the reverse phenotype. Mechanistic studies reveal that miR-100 repressed the activity of a reporter gene fused to the 3'-untranslated region (3'-UTR) of RAP1B, whereas miR-100 silencing upregulated the expression of the reporter gene. Furthermore, we also detected that RAP1B mRNA was inversely expressed with miR-100 in colorectal cancer tissues. These data indicate that the miR-100 plays a tumor suppressor role by regulating colorectal cancer cell growth and invasion phenotype, and could serve as a potential maker for colorectal cancer therapy.

PMID:
24626817
DOI:
10.3892/or.2014.3075
[Indexed for MEDLINE]
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