Format

Send to

Choose Destination
See comment in PubMed Commons below
Methods. 2014 Jun 15;68(1):2-14. doi: 10.1016/j.ymeth.2014.02.037. Epub 2014 Mar 12.

A guide to study Drosophila muscle biology.

Author information

1
Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
2
Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany. Electronic address: schnorrer@biochem.mpg.de.

Abstract

The development and molecular composition of muscle tissue is evolutionarily conserved. Drosophila is a powerful in vivo model system to investigate muscle morphogenesis and function. Here, we provide a short and comprehensive overview of the important developmental steps to build Drosophila body muscle in embryos, larvae and pupae. We describe key methods, including muscle histology, live imaging and genetics, to study these steps at various developmental stages and include simple behavioural assays to assess muscle function in larvae and adults. We list valuable antibodies and fly strains that can be used for these different methods. This overview should guide the reader to choose the best marker or the appropriate method to obtain high quality muscle morphogenesis data in Drosophila.

KEYWORDS:

Development; Dissection; Drosophila; Imaging; Method; Muscle

PMID:
24625467
DOI:
10.1016/j.ymeth.2014.02.037
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center