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J Virol. 2014 May;88(10):5888-93. doi: 10.1128/JVI.00439-14. Epub 2014 Mar 12.

The C-terminal repeat domains of nsP3 from the Old World alphaviruses bind directly to G3BP.

Author information

1
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.

Abstract

The Old World alphaviruses block stress granule assembly by sequestration of RasGAP SH3-domain binding protein (G3BP). Here, we show that the proline-rich sequences in the hypervariable domain of nonstructural protein 3 (nsP3) of both Semliki Forest virus and Chikungunya virus were dispensable for binding to G3BP. nsP3 variants with or without this domain colocalized with G3BP. Furthermore, we show that the C-terminal repeat motifs of nsP3 were sufficient for G3BP binding.

PMID:
24623412
PMCID:
PMC4019107
DOI:
10.1128/JVI.00439-14
[Indexed for MEDLINE]
Free PMC Article

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