Abstract
Yersina pestis, the bubonic plague bacterium, is coated with a polymeric protein hydrogel for protection from host defences. The protein, which is robust and non-stick, resembles structures found in many eukaryotic extracellular-matrix proteins. Cells grown on the natural polymer cannot adhere and grow poorly; however, when cell-adhesion motifs are inserted into the protein, the cells proliferate.
© The Authors, 2014. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adhesiveness
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Animals
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Bacterial Proteins / chemistry*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Biomimetic Materials / chemistry*
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Biomimetic Materials / metabolism
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Cell Adhesion / physiology*
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Cell Proliferation / physiology*
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Extracellular Matrix Proteins / chemistry*
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Extracellular Matrix Proteins / genetics
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Extracellular Matrix Proteins / metabolism
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Humans
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Materials Testing
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Mice
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NIH 3T3 Cells
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Protein Engineering / methods
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Recombinant Fusion Proteins / chemistry
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Structure-Activity Relationship
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Tissue Engineering / methods*
Substances
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Bacterial Proteins
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Extracellular Matrix Proteins
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Recombinant Fusion Proteins
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caf1 protein, Yersinia pestis