Format

Send to

Choose Destination
Methods Mol Biol. 2014;1105:481-95. doi: 10.1007/978-1-62703-739-6_34.

Measuring recombination proficiency in mouse embryonic stem cells.

Author information

1
Markey Cancer Center, University of Kentucky, Lexington, KY, USA.

Abstract

A method is presented to measure homologous recombination in mouse embryonic stem cells by both gene targeting and short-tract gene conversion of a double-strand break (DSB). A fluorescence-based reporter is first gene targeted to the Hprt locus in a quantifiable way. A homing endonuclease expression vector is then introduced to generate a DSB, the repair of which is also quantifiable.

PMID:
24623248
DOI:
10.1007/978-1-62703-739-6_34
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center