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Methods Mol Biol. 2014;1105:457-79. doi: 10.1007/978-1-62703-739-6_33.

The gene cluster instability (GCI) assay for recombination.

Author information

1
Markey Cancer Center, University of Kentucky, Lexington, KY, USA.

Abstract

A newly developed method for quantitatively detecting genomic restructuring in cultured human cell lines as the result of recombination is presented: the "gene cluster instability" (GCI) assay. The assay is physiological in that it detects spontaneous restructuring without the need for exogenous recombination-initiating treatments such as DNA damage. As an assay for genotoxicity, the GCI assay is complementary to well-established sister chromatid exchange (SCE) methods. Analysis of the U-2 OS osteosarcoma cell line is presented as an illustration of the method.

PMID:
24623247
DOI:
10.1007/978-1-62703-739-6_33
[Indexed for MEDLINE]

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