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Methods Mol Biol. 2014;1105:439-55. doi: 10.1007/978-1-62703-739-6_32.

The sister chromatid exchange (SCE) assay.

Author information

1
Markey Cancer Center, University of Kentucky, 222 Combs Research Building, 800 Rose Street, Lexington, KY, 40536-0096, USA.

Abstract

A fully optimized staining method for detecting sister chromatid exchanges in cultured cells is presented. The method gives reproducibly robust quantitative results. Sister chromatid exchange is a classic toxicology assay for genotoxicity and for detecting alterations to the biochemistry underlying cellular homologous recombination. Growth of cells in the presence of 5'-bromo-deoxyuridine for two rounds of DNA replication followed by collecting metaphase spreads on glass slides, treatment with the UV-sensitive dye Hoechst 33258, long-wave UV light exposure, and Giemsa staining gives a permanent record of the exchanges.

PMID:
24623246
DOI:
10.1007/978-1-62703-739-6_32
[Indexed for MEDLINE]

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