Abstract
cDNA molecules encoding the structural proteins of the virulent Trinidad donkey and the TC-83 vaccine strains of Venezuelan equine encephalitis (VEE) virus were inserted under control of the vaccinia virus 7.5K promoter into the thymidine kinase gene of vaccinia virus. Synthesis of the capsid protein and glycoproteins E2 and E1 of VEE virus was demonstrated by immunoblotting of lysates of CV-1 cells infected with recombinant vaccinia/VEE viruses. VEE glycoproteins were detected in recombinant virus-infected cells by fluorescent antibody (FA) analysis performed with a panel of VEE-specific monoclonal antibodies. Seven E2-specific epitopes and two of four E1-specific epitopes were demonstrated by FA.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Antibodies, Monoclonal
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Base Sequence
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Cloning, Molecular
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DNA
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Encephalitis Virus, Venezuelan Equine / genetics*
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Encephalitis Virus, Venezuelan Equine / metabolism
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Epitopes / genetics
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Fluorescent Antibody Technique
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Immunoblotting
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Plasmids
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RNA, Viral
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Recombinant Fusion Proteins / biosynthesis*
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Recombinant Fusion Proteins / genetics
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Recombinant Proteins / biosynthesis*
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Vaccinia virus / genetics*
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Vaccinia virus / metabolism
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Viral Envelope Proteins / biosynthesis
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Viral Envelope Proteins / genetics
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Viral Proteins / biosynthesis*
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Viral Proteins / genetics
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Viral Structural Proteins
Substances
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Antibodies, Monoclonal
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Epitopes
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RNA, Viral
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Recombinant Fusion Proteins
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Recombinant Proteins
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Viral Envelope Proteins
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Viral Proteins
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Viral Structural Proteins
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DNA