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J Infect Dis. 2014 Sep 15;210(6):982-91. doi: 10.1093/infdis/jiu151. Epub 2014 Mar 11.

Group B streptococcal serine-rich repeat proteins promote interaction with fibrinogen and vaginal colonization.

Author information

1
Department of Biology and Center for Microbial Sciences, San Diego State University.
2
Division of Infectious Diseases, Veteran Affairs Medical Center and the University of California San Francisco, California Radiation Biotechnology Research Division, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Republic of Korea.
3
Department of Immunology and Microbiology, School of Medicine, Wayne State University, Detroit, Michigan.
4
Division of Infectious Diseases, Veteran Affairs Medical Center and the University of California San Francisco, California.
5
Department of Biology and Center for Microbial Sciences, San Diego State University Department of Pediatrics, University of California San Diego School of Medicine, La Jolla.

Abstract

Group B streptococcus (GBS) can cause severe disease in susceptible hosts, including newborns, pregnant women, and the elderly. GBS serine-rich repeat (Srr) surface glycoproteins are important adhesins/invasins in multiple host tissues, including the vagina. However, exact molecular mechanisms contributing to their importance in colonization are unknown. We have recently determined that Srr proteins contain a fibrinogen-binding region (BR) and hypothesize that Srr-mediated fibrinogen binding may contribute to GBS cervicovaginal colonization. In this study, we observed that fibrinogen enhanced wild-type GBS attachment to cervical and vaginal epithelium, and that this was dependent on Srr1. Moreover, purified Srr1-BR peptide bound directly to host cells, and peptide administration in vivo reduced GBS recovery from the vaginal tract. Furthermore, a GBS mutant strain lacking only the Srr1 "latching" domain exhibited decreased adherence in vitro and decreased persistence in a mouse model of GBS vaginal colonization, suggesting the importance of Srr-fibrinogen interactions in the female reproductive tract.

KEYWORDS:

GBS; Srr1; fibrinogen; vaginal colonization

PMID:
24620021
PMCID:
PMC4192050
DOI:
10.1093/infdis/jiu151
[Indexed for MEDLINE]
Free PMC Article

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