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Planta Med. 2014 Mar;80(5):399-408. doi: 10.1055/s-0034-1368183. Epub 2014 Mar 11.

Matrine cooperates with all-trans retinoic acid on differentiation induction of all-trans retinoic acid-resistant acute promyelocytic leukemia cells (NB4-LR1): possible mechanisms.

Author information

1
Department of Hematology, Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou, China.
2
Department of Bone Marrow Transplantation, First Affiliated Hospital of Zhejiang University, Hangzhou, China.
3
Zhejiang University of Traditional Chinese Medicine, Hangzhou, China.

Abstract

Retinoic acid resistance results in refractory disease, and recovery in acute promyelocytic leukemia remains a challenge in clinical practice, with no ideal chemotherapeutic drug currently available. Here we report on the effect of an active compound of Sophora flavescens called matrine (0.1 mmol/L) combined with all-trans retinoic acid (1 µmol/L) in alleviating retinoic acid resistance in acute promyelocytic leukemia-derived NB4-LR1 cells by differentiation induction, as can be seen by an induced morphology change, increased CD11b expression, and nitro blue tetrazolium reduction activity, and a decreased expression of the promyelocytic leukemia-retinoic acid receptor α fusion gene and protein product. We further explored the probable mechanism of how matrine promotes the recovery of differentiation ability in NB4-LR1 cells when exposed to all-trans retinoic acid. We observed that the combination of all-trans retinoic acid and matrine can increase the level of cyclic adenosine monophosphate and protein kinase A activity, reduce telomerase activity, and downregulate the protein expression of topoisomerase II beta in NB4-LR1 cells. The results of this study suggest the possible clinical utility of matrine in the treatment of retinoic acid-resistant acute promyelocytic leukemia.

PMID:
24619838
DOI:
10.1055/s-0034-1368183
[Indexed for MEDLINE]
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