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Biomol NMR Assign. 2015 Apr;9(1):81-8. doi: 10.1007/s12104-014-9549-z. Epub 2014 Mar 12.

Assignments of RNase A by ADAPT-NMR and enhancer.

Author information

1
National Magnetic Resonance Facility at Madison, University of Wisconsin - Madison, 433 Babcock Drive, Madison, WI, 53706, USA, tonelli@nmrfam.wisc.edu.

Abstract

We report here backbone (1)H and (15)N assignments for ribonuclease A obtained by using ADAPT-NMR, a fully-automated approach for combined data collection, spectral analysis and resonance assignment. ADAPT-NMR was able to assign 98% of the resonances with 93% agreement with traditional data collection and assignment. Further refinement of the automated results with ADAPT-NMR enhancer led to complete (100%) assignments with 96% agreement with assignments by the traditional approach.

PMID:
24619609
PMCID:
PMC4162851
DOI:
10.1007/s12104-014-9549-z
[Indexed for MEDLINE]
Free PMC Article

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