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Viruses. 2014 Mar 10;6(3):1091-111. doi: 10.3390/v6031091.

Equilibrium and kinetics of Sin Nombre hantavirus binding at DAF/CD55 functionalized bead surfaces.

Author information

1
Department of Pathology, University of New Mexico School of Medicine, MSC08 4640, Albuquerque, NM 87131, USA. buranda@unm.edu.
2
Department of Pathology, University of New Mexico School of Medicine, MSC08 4640, Albuquerque, NM 87131, USA. scswanson@salud.unm.edu.
3
Department of Pathology, University of New Mexico School of Medicine, MSC08 4640, Albuquerque, NM 87131, USA. vbondu@salud.unm.edu.
4
Planet Biotechnology Inc., 25571 Clawiter Road, Hayward, CA 94545, USA. lschaefer@planetbiotechnology.com.
5
Planet Biotechnology Inc., 25571 Clawiter Road, Hayward, CA 94545, USA. jmaclean@planetbiotechnology.com.
6
Planet Biotechnology Inc., 25571 Clawiter Road, Hayward, CA 94545, USA. zmo@planetbiotechnology.com.
7
Planet Biotechnology Inc., 25571 Clawiter Road, Hayward, CA 94545, USA. kwycoff@planetbiotechnology.com.
8
Planet Biotechnology Inc., 25571 Clawiter Road, Hayward, CA 94545, USA. abelle@planetbiotechnology.com.
9
Department of Pathology, University of New Mexico School of Medicine, MSC08 4640, Albuquerque, NM 87131, USA. bhjelle@salud.unm.edu.

Abstract

Decay accelerating factor (DAF/CD55) is targeted by many pathogens for cell entry. It has been implicated as a co-receptor for hantaviruses. To examine the binding of hantaviruses to DAF, we describe the use of Protein G beads for binding human IgG Fc domain-functionalized DAF ((DAF)₂-Fc). When mixed with Protein G beads the resulting DAF beads can be used as a generalizable platform for measuring kinetic and equilibrium binding constants of DAF binding targets. The hantavirus interaction has high affinity (24-30 nM; k(on) ~ 10⁵ M⁻¹ s⁻¹, k(off) ~ 0.0045 s⁻¹). The bivalent (DAF)₂-Fc/SNV data agree with hantavirus binding to DAF expressed on Tanoue B cells (K(d) = 14.0 nM). Monovalent affinity interaction between SNV and recombinant DAF of 58.0 nM is determined from competition binding. This study serves a dual purpose of presenting a convenient and quantitative approach of measuring binding affinities between DAF and the many cognate viral and bacterial ligands and providing new data on the binding constant of DAF and Sin Nombre hantavirus. Knowledge of the equilibrium binding constant allows for the determination of the relative fractions of bound and free virus particles in cell entry assays. This is important for drug discovery assays for cell entry inhibitors.

PMID:
24618810
PMCID:
PMC3970141
DOI:
10.3390/v6031091
[Indexed for MEDLINE]
Free PMC Article

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