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Int Immunol. 2014 Aug;26(8):427-37. doi: 10.1093/intimm/dxu042. Epub 2014 Mar 11.

Role of B-1 cells in the immune response against an antigen encapsulated into phosphatidylcholine-containing liposomes.

Author information

1
Center for Protein Studies, Faculty of Biology, University of Havana, Havana 10400, Cuba.
2
Research and Development Division, Center of Molecular Immunology (CIM), Havana 11600, Cuba.
3
Discipline of Immunology, Department of Microbiology, Immunology and Parasitology, Universidade Federal de São Paulo, 4023-900, SP, Brazil.
4
Department of Microbiology, Immunology and Parasitology, Universidade Federal de São Paulo, 4023-900, SP, Brazil and.
5
Discipline of Immunology, Department of Microbiology, Immunology and Parasitology, Universidade Federal de São Paulo, 4023-900, SP, Brazil Universidade Paulista UNIP, São Paulo, Brazil.
6
Center for Protein Studies, Faculty of Biology, University of Havana, Havana 10400, Cuba mlanio@fbio.uh.cu.

Abstract

B-1 lymphocytes comprise a unique subset of B cells that differ phenotypically, ontogenetically and functionally from conventional B-2 cells. A frequent specificity of the antibody repertoire of peritoneal B-1 cells is phosphatidylcholine. Liposomes containing phosphatidylcholine have been studied as adjuvants and their interaction with dendritic cells and macrophages has been demonstrated. However, the role of B-1 cells in the adjuvanticity of liposomes composed of phosphatidylcholine has not been explored. In the present work, we studied the contribution of B-1 cells to the humoral response against ovalbumin (OVA) encapsulated into dipalmitoylphosphatidylcholine (DPPC) and cholesterol-containing liposomes. BALB/X-linked immunodeficient (xid) mice, which are deficient in B-1 cells, showed quantitative and qualitative differences in the anti-OVA antibody response compared with wild-type animals after immunization with these liposomes. The OVA-specific immune response was significantly increased in the BALB/xid mice when reconstituted with B-1 cells from naive BALB/c mice. Our results indicate the internalization of DPPC-containing liposomes by these cells and their migration from the peritoneal cavity to the spleen. Phosphatidylcholine significantly contributed to the immunogenicity of liposomes, as DPPC-containing liposomes more effectively stimulated the anti-OVA response compared with vesicles composed of dipalmitoylphosphatidylglycerol. In conclusion, we present evidence for a cognate interaction between B-1 cells and phosphatidylcholine liposomes, modulating the immune response to encapsulated antigens. This provides a novel targeting approach to assess the role of B-1 cells in humoral immunity.

KEYWORDS:

B-1 lymphocytes; adjuvants; humoral response

PMID:
24618118
DOI:
10.1093/intimm/dxu042
[Indexed for MEDLINE]

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