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Small. 2014 Jul 23;10(14):2877-82. doi: 10.1002/smll.201303325. Epub 2014 Mar 10.

On-chip detection of rolling circle amplified DNA molecules from Bacillus globigii spores and Vibrio cholerae.

Author information

1
Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Building 345 East, DK-2800, Kongens Lyngby, Denmark.

Abstract

For the first time DNA coils formed by rolling circle amplification are quantified on-chip by Brownian relaxation measurements on magnetic nanobeads using a magnetoresistive sensor. No external magnetic fields are required besides the magnetic field arising from the current through the sensor, which makes the setup very compact. Limits of detection down to 500 Bacillus globigii spores and 2 pM of Vibrio cholerae are demonstrated, which are on the same order of magnitude or lower than those achieved previously using a commercial macro-scale AC susceptometer. The chip-based readout is an important step towards the realization of field tests based on rolling circle amplification molecular analyses.

KEYWORDS:

biosensors; brownian relaxation; lab-on-a-chip; magnetic field sensors; magnetic nanoparticles

PMID:
24616417
DOI:
10.1002/smll.201303325
[Indexed for MEDLINE]

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