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Chembiochem. 2014 Apr 14;15(6):826-8. doi: 10.1002/cbic.201300602. Epub 2014 Mar 11.

Rapid determination of the amino acid configuration of xenotetrapeptide.

Author information

1
Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Goethe Universität Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main (Germany).

Abstract

An E. coli strain with deletions in five transaminases (ΔaspC ΔilvE ΔtyrB ΔavtA ΔybfQ) was constructed to be unable to degrade several amino acids. This strain was used as an expression host for the analysis of the amino acid configuration of nonribosomally synthesized peptides, including the novel peptide "xenotetrapeptide" from Xenorhabdus nematophila, by using a combination of labeling experiments and mass spectrometry. Additionally, the number of D-amino acids in the produced peptide was assigned following simple cultivation of the expression strain in D2 O.

KEYWORDS:

D-amino acids; absolute configuration; deuterium exchange; mass spectrometry; peptides; structure elucidation

PMID:
24616055
DOI:
10.1002/cbic.201300602
[Indexed for MEDLINE]

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