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Sci Rep. 2014 Mar 11;4:4347. doi: 10.1038/srep04347.

Changes in IgG and total plasma protein glycomes in acute systemic inflammation.

Author information

1
Genos Glycoscience Laboratory, Zagreb, Croatia.
2
Department of Orthopaedic Surgery University Hospital Centre Zagreb, Croatia.
3
Clinic for Cardiac Surgery, University Hospital Centre Zagreb, Croatia.
4
University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Biochemistry and Molecular Biology, Zagreb, Croatia.
5
Department of Physics and Astronomy DIFA, University of Bologna, Bologna, Italy.
6
1] University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Biochemistry and Molecular Biology, Zagreb, Croatia [2].
7
1] Genos Glycoscience Laboratory, Zagreb, Croatia [2] University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Biochemistry and Molecular Biology, Zagreb, Croatia [3].

Abstract

Recovery after cardiac surgery is a complex process that has to compensate for both individual variability and extensive tissue damage in the context of systemic inflammation. Protein glycosylation is essential in many steps of the inflammatory cascade, but due to technological limitations the role of individual variation in glycosylation in systemic inflammation has not been addressed until now. We analysed composition of the total plasma and IgG N-glycomes in 107 patients undergoing cardiac surgery. In nearly all individuals plasma N-glycome underwent the same pattern of changes in the first 72 h, revealing a general mechanism of glycosylation changes. To the contrary, changes in the IgG glycome were very individualized. Bi-clustering analysis revealed the existence of four distinct patterns of changes. One of them, characterized by a rapid increase in galactosylated glycoforms, was associated with nearly double mortality risk measured by EuroSCORE II. Our results indicate that individual variation in IgG glycosylation changes during acute systemic inflammation associates with increased mortality risk and indicates new avenues for the development of personalized diagnostic and therapeutic approach.

PMID:
24614541
PMCID:
PMC3949295
DOI:
10.1038/srep04347
[Indexed for MEDLINE]
Free PMC Article

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