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PLoS One. 2014 Mar 10;9(3):e90089. doi: 10.1371/journal.pone.0090089. eCollection 2014.

Pharmacological inhibition of cochlear mitochondrial respiratory chain induces secondary inflammation in the lateral wall: a potential therapeutic target for sensorineural hearing loss.

Author information

1
Department of Otolaryngology, Head and Neck Surgery, Keio University, School of Medicine, Shinjuku, Tokyo, Japan; Department of Physiology, School of Medicine, Keio University, School of Medicine, Shinjuku, Tokyo, Japan.
2
Department of Otorhinolaryngology, Inagi Municipal Hospital, Inagi, Tokyo, Japan; The Laboratory of Auditory Disorders and Division of Hearing and Balance Research, National Institute of Sensory Organs, National Tokyo Medical Center, Meguro, Tokyo, Japan.
3
Department of Otolaryngology, Saiseikai Utsunomiya Hospital, Utsunomiya, Tochigi, Japan; The Laboratory of Auditory Disorders and Division of Hearing and Balance Research, National Institute of Sensory Organs, National Tokyo Medical Center, Meguro, Tokyo, Japan.
4
Department of Physiology, School of Medicine, Keio University, School of Medicine, Shinjuku, Tokyo, Japan; Division of Regenerative Medicine, Jikei University School of Medicine, Tokyo, Japan.
5
Department of Physiology, School of Medicine, Keio University, School of Medicine, Shinjuku, Tokyo, Japan.
6
Department of Otolaryngology, Head and Neck Surgery, Keio University, School of Medicine, Shinjuku, Tokyo, Japan.
7
The Laboratory of Auditory Disorders and Division of Hearing and Balance Research, National Institute of Sensory Organs, National Tokyo Medical Center, Meguro, Tokyo, Japan.

Abstract

Cochlear lateral wall has recently been reported as a common site of inflammation, yet precise molecular mechanisms of the inflammatory responses remain elucidated. The present study examined the inflammatory responses in the lateral wall following acute mitochondrial dysfunction induced by a mitochondrial toxin, 3-nitropropionic acid (3-NP). Reverse-transcription (RT)-PCR revealed increases in the expression of the proinflammatory cytokines interleukin (IL)-1β and IL-6. Immunohistochemistry showed an increase in the number of activated cochlear macrophages in the lateral wall, which were in close proximity to IL-6-expressing cells. A genome-wide DNA microarray analysis of the lateral wall revealed that 35% and 60% of the genes showing >2-fold upregulation at 1 d and 3 d post-3-NP administration, respectively, were inflammatory genes, including CC- and CXC-type chemokine genes. High expression of CCL-1, 2, and 3 at 1 d, and of CCL-1, 2, 3, 4, and 5, CCR-2 and 5, and CX3CR1 at 3 d post-3-NP administration, coupled with no change in the level of CX3CL1 expression suggested that macrophages and monocytes may be involved in the inflammatory response to 3-NP-mediated injury. Quantitative (q)RT-PCR showed a transient induction of IL-1β and IL-6 expression within 24 h of 3-NP-mediated injury, followed by sustained expression of the chemoattractants, CCL-2, 4 and 5, up until 7 d after injury. The expression of CCL-2 and IL-6 was higher in animals showing permanent hearing impairment than in those showing temporary hearing impairment, suggesting that these inflammatory responses may be detrimental to hearing recovery. The present findings suggest that acute mitochondrial dysfunction induces secondary inflammatory responses in the lateral wall of the cochlear and that the IL-6/CCL-2 inflammatory pathway is involved in monocyte activation. Therefore, these secondary inflammatory responses may be a potential post-insult therapeutic target for treatments aimed at preventing the damage caused by acute mitochondrial dysfunction in the cochlear lateral wall.

PMID:
24614528
PMCID:
PMC3948682
DOI:
10.1371/journal.pone.0090089
[Indexed for MEDLINE]
Free PMC Article

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