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PLoS One. 2014 Mar 10;9(3):e90588. doi: 10.1371/journal.pone.0090588. eCollection 2014.

A pepper MSRB2 gene confers drought tolerance in rice through the protection of chloroplast-targeted genes.

Author information

1
Division of Bioscience and Bioinformatics, Myong Ji University, Yongin, Kyonggido, Korea.
2
Rice research division, National Institute of Crop Science, Suwon, Korea.
3
Genomics Genetics Institute, GreenGene BioTech Inc., Yongin, Kyonggido, Korea.
4
National Academy of Agricultural Science, Rural Development Administration, Suwon, Korea.
5
Functional Food Center, Korea Institute of Science and Technology (KIST), Gangneung, Gangwon-do, Korea.
6
College of Agriculture and Life Sciences and Plant Genomics & Breeding Institute, Seoul National University, Seoul, Korea.
7
Division of Bioscience and Bioinformatics, Myong Ji University, Yongin, Kyonggido, Korea; Genomics Genetics Institute, GreenGene BioTech Inc., Yongin, Kyonggido, Korea.

Abstract

BACKGROUND:

The perturbation of the steady state of reactive oxygen species (ROS) due to biotic and abiotic stresses in a plant could lead to protein denaturation through the modification of amino acid residues, including the oxidation of methionine residues. Methionine sulfoxide reductases (MSRs) catalyze the reduction of methionine sulfoxide back to the methionine residue. To assess the role of this enzyme, we generated transgenic rice using a pepper CaMSRB2 gene under the control of the rice Rab21 (responsive to ABA protein 21) promoter with/without a selection marker, the bar gene.

RESULTS:

A drought resistance test on transgenic plants showed that CaMSRB2 confers drought tolerance to rice, as evidenced by less oxidative stress symptoms and a strengthened PSII quantum yield under stress conditions, and increased survival rate and chlorophyll index after the re-watering. The results from immunoblotting using a methionine sulfoxide antibody and nano-LC-MS/MS spectrometry suggest that porphobilinogen deaminase (PBGD), which is involved in chlorophyll synthesis, is a putative target of CaMSRB2. The oxidized methionine content of PBGD expressed in E. coli increased in the presence of H2O2, and the Met-95 and Met-227 residues of PBGD were reduced by CaMSRB2 in the presence of dithiothreitol (DTT). An expression profiling analysis of the overexpression lines also suggested that photosystems are less severely affected by drought stress.

CONCLUSIONS:

Our results indicate that CaMSRB2 might play an important functional role in chloroplasts for conferring drought stress tolerance in rice.

PMID:
24614245
PMCID:
PMC3948683
DOI:
10.1371/journal.pone.0090588
[Indexed for MEDLINE]
Free PMC Article

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