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Methods. 2014 Jun 15;68(1):242-51. doi: 10.1016/j.ymeth.2014.02.038. Epub 2014 Mar 12.

Drosophila hematopoiesis: Markers and methods for molecular genetic analysis.

Author information

1
Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
2
Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: banerjee@mbi.ucla.edu.

Abstract

Analyses of the Drosophila hematopoietic system are becoming more and more prevalent as developmental and functional parallels with vertebrate blood cells become more evident. Investigative work on the fly blood system has, out of necessity, led to the identification of new molecular markers for blood cell types and lineages and to the refinement of useful molecular genetic tools and analytical methods. This review briefly describes the Drosophila hematopoietic system at different developmental stages, summarizes the major useful cell markers and tools for each stage, and provides basic protocols for practical analysis of circulating blood cells and of the lymph gland, the larval hematopoietic organ.

KEYWORDS:

Blood; Drosophila; Hematopoiesis; Hemocytes; Lymph gland; Protocol

PMID:
24613936
PMCID:
PMC4051208
DOI:
10.1016/j.ymeth.2014.02.038
[Indexed for MEDLINE]
Free PMC Article

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