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Methods. 2014 Jun 15;68(1):89-96. doi: 10.1016/j.ymeth.2014.02.033. Epub 2014 Mar 6.

Detecting apoptosis in Drosophila tissues and cells.

Author information

1
CBRC, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02129, USA.
2
Department of Biology, Boston University, Boston, MA 02215, USA.
3
CBRC, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02129, USA. Electronic address: kristin.white@cbrc2.mgh.harvard.edu.

Abstract

In this chapter we discuss methods that can be used to study apoptotic cell death in the Drosophila embryo, ovary, as well as in cultured cell lines. These methods assay various aspects of the cell death process, from mitochondrial changes to caspase activation and DNA cleavage. The assays are useful for examining apoptosis in normal development and in response to developmental perturbations and external stresses. These techniques include Acridine Orange staining, TUNEL, cleaved caspase staining, caspase activity assays and assays for mitochondrial fission and permeabilization.

KEYWORDS:

Apoptosis; Caspase; Drosophila; TUNEL

PMID:
24613678
PMCID:
PMC4048790
DOI:
10.1016/j.ymeth.2014.02.033
[Indexed for MEDLINE]
Free PMC Article

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