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Biomaterials. 2014 May;35(15):4525-4535. doi: 10.1016/j.biomaterials.2014.02.008. Epub 2014 Mar 5.

The effect of delivering the chemokine SDF-1α in a matrix-bound manner on myogenesis.

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CNRS UMR 5628 (LMGP), 3 parvis Louis Néel, 38016 Grenoble, France.
Université Grenoble Alpes, LMGP, 3 parvis Louis Néel, 38016 Grenoble, France.
FONDATION ARC, 9 rue Guy Môquet 94803 Villejuif, France.
Univ. Grenoble Alpes, Institut de Biologie Structurale (IBS), F-38027 Grenoble, France.
CNRS, IBS, F-38027 Grenoble, France.
CEA, DSV, IBS, F-38027 Grenoble, France.
Centre de Recherche en Cancérologie de Lyon, UMR INSERM 1052 - CNRS 5286, 28, rue Laennec, 69373 LYON cedex 08, France.
Inserm U823, ERL CNRS5284, Université Joseph Fourier, Institut Albert Bonniot, Site Santé, BP170, 38042 Grenoble cedex 9, France.
Contributed equally


Several chemokines are important in muscle myogenesis and in the recruitment of muscle precursors during muscle regeneration. Among these, the SDF-1α chemokine (CXCL12) is a potent chemoattractant known to be involved in muscle repair. SDF-1α was loaded in polyelectrolyte multilayer films made of poly(L-lysine) and hyaluronan to be delivered locally to myoblast cells in a matrix-bound manner. The adsorbed amounts of SDF-1α were tuned over a large range from 100 ng/cm(2) to 5 μg/cm(2), depending on the initial concentration of SDF-1α in solution, its pH, and on the film crosslinking extent. Matrix-bound SDF-1α induced a striking increase in myoblast spreading, which was revealed when it was delivered from weakly crosslinked films. It also significantly enhanced cell migration in a dose-dependent manner, which again depended on its presentation by the biopolymeric film. The low-crosslinked film was the most efficient in boosting cell migration. Furthermore, matrix-bound SDF-1α also increased the expression of myogenic markers but the fusion index decreased in a dose-dependent manner with the adsorbed amount of SDF-1α. At high adsorbed amounts of SDF-1α, a large number of Troponin T-positive cells had only one nucleus. Overall, this work reveals the importance of the presentation mode of SDF-1α to emphasize its effect on myogenic processes. These films may be further used to provide insight into the role of SDF-1α presented by a biomaterial in physiological or pathological processes.


CXCR4; Drug delivery; Migration; Myoblast; Polyelectrolyte multilayer film; SDF-1α/CXCL12

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