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J Vet Intern Med. 2014 Mar-Apr;28(2):564-70. doi: 10.1111/jvim.12330. Epub 2014 Feb 24.

Immunohistochemical expression of potential therapeutic targets in canine thyroid carcinoma.

Author information

1
Department of Medicine and Clinical Biology of Small Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.

Abstract

BACKGROUND:

Thyroid carcinoma is a common endocrine tumor in the dog. Local invasive growth frequently precludes surgical excision and, in up to 38% of dogs, the tumor has already metastasized by the time of diagnosis. Therefore, it is important to investigate new treatment modalities that may be useful for the large number of dogs with inoperable tumors or metastatic disease.

HYPOTHESIS/OBJECTIVES:

To investigate the immunohistochemical expression of potential therapeutic targets in canine thyroid tumors.

ANIMALS:

74 dogs with thyroid neoplasia.

METHODS:

Immunohistochemistry was performed for thyroglobulin, calcitonin, vascular endothelial growth factor (VEGF), p53, cycloxygenase-2 (cox-2), and P-glycoprotein (P-gp).

RESULTS:

Fifty-four (73%) tumors were classified as follicular cell thyroid carcinomas (FTCs) and 20 (27%) as medullary thyroid carcinomas (MTCs). Eighty percent of FTCs and all MTCs had a high percentage (76-100%) of neoplastic cells immunopositive for VEGF. Thirteen percent of FTCs and 50% of MTCs expressed cox-2. Seven percent of FTCs and 70% of MTCs expressed P-gp. No tumor was immunopositive for p53 expression. Expression of VEGF (P = .034), cox-2 (P = .013), and P-gp (P < .001) was significantly higher in MTCs compared to FTCs.

CONCLUSIONS AND CLINICAL IMPORTANCE:

VEGF is a potential therapeutic target in both FTC and MTC in dogs. Cox-2 and P-gp may be useful molecular targets in canine MTC.

KEYWORDS:

Cyclooxygenase-2; P-glycoprotein; VEGF; p53

PMID:
24612088
PMCID:
PMC4857974
DOI:
10.1111/jvim.12330
[Indexed for MEDLINE]
Free PMC Article

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