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Nucleic Acids Res. 2014 May;42(9):e75. doi: 10.1093/nar/gku181. Epub 2014 Mar 7.

Estimating telomere length from whole genome sequence data.

Author information

1
Genome Informatics, Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK.
2
Department of Twin Research and Genetic Epidemiology, King's College London, London, WC2R 2LS, UK.
3
The Center for Human Development and Aging, New Jersey Medical School, Rutgers University, Newark, NJ 07103, USA.
4
Genome Informatics, Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK rd@sanger.ac.uk.

Abstract

Telomeres play a key role in replicative ageing and undergo age-dependent attrition in vivo. Here, we report a novel method, TelSeq, to measure average telomere length from whole genome or exome shotgun sequence data. In 260 leukocyte samples, we show that TelSeq results correlate with Southern blot measurements of the mean length of terminal restriction fragments (mTRFs) and display age-dependent attrition comparably well as mTRFs.

PMID:
24609383
PMCID:
PMC4027178
DOI:
10.1093/nar/gku181
[Indexed for MEDLINE]
Free PMC Article

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