Format

Send to

Choose Destination
Nat Chem Biol. 2014 Apr;10(4):286-90. doi: 10.1038/nchembio.1477. Epub 2014 Mar 9.

Dissecting motility signaling through activation of specific Src-effector complexes.

Author information

1
1] Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. [2].
2
Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
3
Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
4
Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
5
1] Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. [2] Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.

Erratum in

  • Nat Chem Biol. 2014 Aug;10(8):692.

Abstract

We describe an approach to selectively activate a kinase in a specific protein complex or at a specific subcellular location within living cells and within minutes. This reveals the effects of specific kinase pathways without time for genetic compensation. The new technique, dubbed rapamycin-regulated targeted activation of pathways (RapRTAP), was used to dissect the role of Src kinase interactions with FAK and p130Cas in cell motility and morphodynamics. The overall effects of Src activation on cell morphology and adhesion dynamics were first quantified, without restricting effector access. Subsets of Src-induced behaviors were then attributed to specific interactions between Src and the two downstream proteins. Activation of Src in the cytoplasm versus at the cell membrane also produced distinct phenotypes. The conserved nature of the kinase site modified for RapRTAP indicates that the technique can be applied to many kinases.

PMID:
24609359
PMCID:
PMC4064790
DOI:
10.1038/nchembio.1477
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center