Format

Send to

Choose Destination
Nat Chem Biol. 2014 Apr;10(4):259-65. doi: 10.1038/nchembio.1476. Epub 2014 Mar 9.

Expanding ester biosynthesis in Escherichia coli.

Author information

1
1] Department of Chemistry, University of California-Davis, Davis, California, USA. [2].
2
Department of Chemistry, University of California-Davis, Davis, California, USA.

Abstract

To expand the capabilities of whole-cell biocatalysis, we have engineered Escherichia coli to produce various esters. The alcohol O-acyltransferase (ATF) class of enzyme uses acyl-CoA units for ester formation. The release of free CoA upon esterification with an alcohol provides the free energy to facilitate ester formation. The diversity of CoA molecules found in nature in combination with various alcohol biosynthetic pathways allows for the biosynthesis of a multitude of esters. Small to medium volatile esters have extensive applications in the flavor, fragrance, cosmetic, solvent, paint and coating industries. The present work enables the production of these compounds by designing several ester pathways in E. coli. The engineered pathways generated acetate esters of ethyl, propyl, isobutyl, 2-methyl-1-butyl, 3-methyl-1-butyl and 2-phenylethyl alcohols. In particular, we achieved high-level production of isobutyl acetate from glucose (17.2 g l(-1)). This strategy was expanded to realize pathways for tetradecyl acetate and several isobutyrate esters.

PMID:
24609358
PMCID:
PMC4411949
DOI:
10.1038/nchembio.1476
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center