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Nat Methods. 2014 May;11(5):555-8. doi: 10.1038/nmeth.2882. Epub 2014 Mar 9.

Single-molecule motions enable direct visualization of biomolecular interactions in solution.

Author information

1
1] Department of Chemistry, Stanford University, Stanford, California, USA. [2] Department of Electrical Engineering, Stanford University, Stanford, California, USA.
2
Department of Chemistry, Stanford University, Stanford, California, USA.

Abstract

Biomolecular interactions are generally accompanied by modifications in size and charge of biomolecules at the nanometer scale. Here we describe a single-molecule method to sense these changes in real time based on statistical learning of diffusive and electric field-induced motion parameters of a trapped molecule in solution. We demonstrate the approach by resolving a monomer-trimer mixture along a protein dissociation pathway and visualizing the binding-unbinding kinetics of a single DNA molecule.

PMID:
24608179
DOI:
10.1038/nmeth.2882
[Indexed for MEDLINE]

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