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Dev Biol. 2014 Jun 1;390(1):41-50. doi: 10.1016/j.ydbio.2014.02.021. Epub 2014 Mar 4.

Exocyst-mediated membrane trafficking is required for branch outgrowth in Drosophila tracheal terminal cells.

Author information

1
Department of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA.
2
Department of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA. Electronic address: markm@genetics.utah.edu.

Abstract

Branching morphogenesis, the process by which cells or tissues generate tree-like networks that function to increase surface area or in contacting multiple targets, is a common developmental motif in multicellular organisms. We use Drosophila tracheal terminal cells, a component of the insect respiratory system, to investigate branching morphogenesis that occurs at the single cell level. Here, we show that the exocyst, a conserved protein complex that facilitates docking and tethering of vesicles at the plasma membrane, is required for terminal cell branch outgrowth. We find that exocyst-deficient terminal cells have highly truncated branches and show an accumulation of vesicles within their cytoplasm and are also defective in subcellular lumen formation. We also show that vesicle trafficking pathways mediated by the Rab GTPases Rab10 and Rab11 are redundantly required for branch outgrowth. In terminal cells, the PAR-polarity complex is required for branching, and we find that the PAR complex is required for proper membrane localization of the exocyst, thus identifying a molecular link between the branching and outgrowth programs. Together, our results suggest a model where exocyst mediated vesicle trafficking facilitates branch outgrowth, while de novo branching requires cooperation between the PAR and exocyst complexes.

KEYWORDS:

Branching morphogenesis; Drosophila; Exocyst complex; Lumenogenesis; PAR complex; Terminal cells; Trachea

PMID:
24607370
PMCID:
PMC4041209
DOI:
10.1016/j.ydbio.2014.02.021
[Indexed for MEDLINE]
Free PMC Article
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