Format

Send to

Choose Destination
See comment in PubMed Commons below
J Infect Dis. 2014 Jun 15;209(12):1860-9. doi: 10.1093/infdis/jiu123. Epub 2014 Mar 5.

A live attenuated influenza A(H5N1) vaccine induces long-term immunity in the absence of a primary antibody response.

Author information

1
Center For Immunization Research, Johns Hopkins Bloomberg School of Public Health, Baltimore.
2
Laboratory of Infectious Diseases.
3
Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland.
4
PATH, Seattle, Washington.
5
Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health.

Erratum in

  • J Infect Dis. 2014 Dec 15;210(12):2021.

Abstract

BACKGROUND:

Highly pathogenic avian influenza A(H5N1) causes severe infections in humans. We generated 2 influenza A(H5N1) live attenuated influenza vaccines for pandemic use (pLAIVs), but they failed to elicit a primary immune response. Our objective was to determine whether the vaccines primed or established long-lasting immunity that could be detected by administration of inactivated subvirion influenza A(H5N1) vaccine (ISIV).

METHODS:

The following groups were invited to participate in the study: persons who previously received influenza A(H5N1) pLAIV; persons who previously received an irrelevant influenza A(H7N3) pLAIV; and community members who were naive to influenza A(H5N1) and LAIV. LAIV-experienced subjects received a single 45-μg dose of influenza A(H5N1) ISIV. Influenza A(H5N1)- and LAIV-naive subjects received either 1 or 2 doses of ISIV.

RESULTS:

In subjects who had previously received antigenically matched influenza A(H5N1) pLAIV followed by 1 dose of ISIV compared with those who were naive to influenza A(H5N1) and LAIV and received 2 doses of ISIV, we observed an increased frequency of antibody response (82% vs 50%, by the hemagglutination inhibition assay) and a significantly higher antibody titer (112 vs 76; P = .04). The affinity of antibody and breadth of cross-clade neutralization was also enhanced in influenza A(H5N1) pLAIV-primed subjects.

CONCLUSIONS:

ISIV administration unmasked long-lasting immunity in influenza A(H5N1) pLAIV recipients, with a rapid, high-titer, high-quality antibody response that was broadly cross-reactive across several influenza A(H5N1) clades.

CLINICAL TRIALS REGISTRATION:

NCT01109329.

KEYWORDS:

H5N1; avian influenza; live attenuated; vaccine

PMID:
24604819
PMCID:
PMC4047295
DOI:
10.1093/infdis/jiu123
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Silverchair Information Systems Icon for PubMed Central
    Loading ...
    Support Center