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Front Genet. 2014 Feb 20;5:31. doi: 10.3389/fgene.2014.00031. eCollection 2014.

Multi-genome alignment for quality control and contamination screening of next-generation sequencing data.

Author information

1
Genomics Core Facility, Cancer Research UK Cambridge institute, University of Cambridge Cambridge, UK.
2
Bioinformatics Core Facility, Cancer Research UK Cambridge institute, University of Cambridge Cambridge, UK.

Abstract

The availability of massive amounts of DNA sequence data, from 1000s of genomes even in a single project has had a huge impact on our understanding of biology, but also creates several problems for biologists carrying out those experiments. Bioinformatic analysis of sequence data is perhaps the most obvious challenge but upstream of this even basic quality control of sequence run performance is challenging for many users given the volume of data. Users need to be able to assess run quality efficiently so that only high-quality data are passed through to computationally-, financially-, and time-intensive processes. There is a clear need to make human review of sequence data as efficient as possible. The multi-genome alignment tool presented here presents next-generation sequencing run data in visual and tabular formats simplifying assessment of run yield and quality, as well as presenting some sample-based quality metrics and screening for contamination from adapter sequences and species other than the one being sequenced.

KEYWORDS:

contamination screen; next-generation sequencing; quality control

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