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Integr Biol (Camb). 2014 Apr;6(4):430-7. doi: 10.1039/c3ib40224j. Epub 2014 Mar 5.

A quantum dot-based microfluidic multi-window platform for quantifying the biomarkers of breast cancer cells.

Author information

1
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. jekyun@kaist.ac.kr.

Abstract

Conventional molecular profiling methods using immunochemical assays have limits in terms of multiplexity and the quantification of biomarkers in investigation of cancer cells. In this paper, we demonstrate a quantum dot (QD)-based microfluidic multiple biomarker quantification (QD-MMBQ) method that enables labeling of more than eight proteins immunochemically on cell blocks within 1 h, in a quantitative manner. An internal reference, β-actin, was used as a loading control to compensate for differences in not only the cell number but also in staining quality among specimens. Furthermore, the microfluidic blocking method exhibited less nonspecific binding of QDs than the conventional static blocking method.

PMID:
24599518
DOI:
10.1039/c3ib40224j
[Indexed for MEDLINE]

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