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ISME J. 2014 Sep;8(9):1949-52. doi: 10.1038/ismej.2014.29. Epub 2014 Mar 6.

Intragenus generalized transduction in Staphylococcus spp. by a novel giant phage.

Author information

1
1] Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kochi, Japan [2] Center for Innovative and Translational Medicine, Faculty of Medicine, Kochi University, Kochi, Japan.
2
Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kochi, Japan.
3
Interdisciplinary Research Organization, University of Miyazaki, Miyazaki, Japan.
4
Research and Education Faculty, Multidisciplinary Science Cluster, Interdisciplinary Science Unit, Kochi University, Kochi, Japan.
5
Research Institute of Molecular Genetics, Kochi University, Kochi, Japan.
6
Science Research Center, Kochi University, Kochi, Japan.
7
Laboratory of Veterinary Public Health, Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.

Abstract

Bacteriophage (phage)-mediated generalized transduction is expected to contribute to the emergence of drug-resistant staphylococcal clones in various environments. In this study, novel phage S6 was isolated from sewage and used to test generalized transduction in human- and animal-derived staphylococci. Phage S6 was a novel type of giant myophage, which possessed a DNA genome that contained uracil instead of thymine, and it could infect all of the tested staphylococcal species. The phage S6 appeared to be similar to the transducing phage PBS1, which infects Bacillus spp. Moreover, phage S6 facilitated the transduction of a plasmid in Staphylococcus aureus and from S. aureus to non-aureus staphylococcal species, as well as vice versa. Transduction of methicillin resistance also occurred in S. aureus. This is the first report of successful intragenus generalized transduction among staphylococci.

PMID:
24599069
PMCID:
PMC4139722
DOI:
10.1038/ismej.2014.29
[Indexed for MEDLINE]
Free PMC Article

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