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Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):685-93. doi: 10.1107/S1399004713032392. Epub 2014 Feb 15.

Exploiting large non-isomorphous differences for phase determination of a G-segment invertase-DNA complex.

Author information

1
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.

Abstract

Crystals of the G-segment invertase in complex with a 37-base-pair asymmetric DNA duplex substrate had an unusually high solvent content of 88% and diffracted to a maximal resolution of about 5.0 Å. These crystals exhibited a high degree of non-isomorphism and anisotropy, which presented a serious challenge for structure determination by isomorphous replacement. Here, a procedure of cross-crystal averaging is described that uses large non-isomorphous crystallographic data with a priori information of an approximate molecular boundary as determined from a minimal amount of experimental phase information. Using this procedure, high-quality experimental phases were obtained that have enabled it to be shown that the conformation of the bound substrate DNA duplex significantly differs from those of substrates bound in other serine recombinase-DNA complexes.

KEYWORDS:

DNA; G-segment invertase; cross-crystal averaging; non-isomorphism

PMID:
24598738
PMCID:
PMC3949525
DOI:
10.1107/S1399004713032392
[Indexed for MEDLINE]
Free PMC Article
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