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Zygote. 2015 Jun;23(3):412-5. doi: 10.1017/S096719941400001X. Epub 2014 Mar 5.

The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development.

Author information

1
Institute: Laboratory of Theriogenology,Department of Veterinary Clinical Sciences,Graduate School of Veterinary Medicine,Hokkaido University,Sapporo 060-0818,Japan.
2
National Livestock Breeding Center,Nishigo,Fukushima 961-8511,Japan.
3
Laboratory of Theriogenology,Department of Veterinary Clinical Sciences,Graduate School of Veterinary Medicine,Hokkaido University,Kita-ku Kita 18 Nishi 9,Sapporo 060-0818,Japan.

Abstract

The aim of the present study was to clarify the efficacy of the well of the well (WOW) culture system for a small number of embryos and the effect of number of adjacent embryos in a WOW dish on blastocyst development. In conventional droplet culture, embryos in the small-number group (5-6 embryos/droplet) showed low blastocyst development compared with a control group (25-26 embryos/droplet). However, small and large numbers of embryos (5-6 and 25 embryos, respectively) in a WOW dish showed no significant differences in cleavage, blastocyst rates, and mean cell number in blastocysts compared with the control group (25-30 embryos/droplet). In addition, the number of adjacent embryos in a WOW dish did not affect the development to blastocysts and cell number in blastocysts. In conclusion, a WOW dish can provide high and stable blastocyst development in small group culture wherever embryos are placed in microwells of the WOW dish.

KEYWORDS:

WOW

PMID:
24598303
DOI:
10.1017/S096719941400001X
[Indexed for MEDLINE]
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