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Avian Dis. 2013 Dec;57(4):797-9.

Goose Hemorrhagic polyomavirus detection in geese using real-time PCR assay.

Author information

1
Université de Toulouse, Institut National Polytechnique, Ecole Nationale Vétérinaire de Toulouse, 23, chemin des Capelles, F-31076 Toulouse, France.

Abstract

Goose hemorrhagic polyomavirus (GHPV) is the viral agent of hemorrhagic nephritis enteritis of geese (HNEG), a lethal disease of goslings. Although death is the most common outcome, geese that recover from HNEG are persistently infected. Here, we present the development of real-time SYBR Green real-time PCR targeted to GHPV and its use to assess the prevalence of GHPV infection in French geese flocks. When compared with classical end-point PCR, real-time PCR revealed a much better sensitivity and equivalent specificity. Real-time PCR could, therefore, be considered a gold standard for the detection of GHPV. Results of field investigations evidenced a very high prevalence of GHPV infections in French geese, largely associated with healthy carriage.

PMID:
24597124
DOI:
10.1637/10513-021013-ResNote.1
[Indexed for MEDLINE]

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