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Environ Microbiol Rep. 2014 Feb;6(1):21-7. doi: 10.1111/1758-2229.12104. Epub 2013 Nov 10.

Successful enrichment of the ubiquitous freshwater acI Actinobacteria.

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1
Jena School for Microbial Communication (JSMC) and Microbial Ecology Group at Friedrich Schiller University, Jena, Germany.

Abstract

Actinobacteria of the acI lineage are often the numerically dominant bacterial phylum in surface freshwaters, where they can account for > 50% of total bacteria. Despite their abundance, there are no described isolates. In an effort to obtain enrichment of these ubiquitous freshwater Actinobacteria, diluted freshwater samples from Lake Grosse Fuchskuhle, Germany, were incubated in 96-well culture plates. With this method, a successful enrichment containing high abundances of a member of the lineage acI was established. Phylogenetic classification showed that the acI Actinobacteria of the enrichment belonged to the acI-B2 tribe, which seems to prefer acidic lakes. This enrichment grows to low cell densities and thus the oligotrophic nature of acI-B2 was confirmed.

PMID:
24596259
DOI:
10.1111/1758-2229.12104
[Indexed for MEDLINE]
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