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Elife. 2014 Jan 1;3:e01879. doi: 10.7554/eLife.01879.

Membranes linked by trans-SNARE complexes require lipids prone to non-bilayer structure for progression to fusion.

Author information

1
Department of Biochemistry, Geisel School of Medicine at Dartmouth, Hanover, United States.

Abstract

Like other intracellular fusion events, the homotypic fusion of yeast vacuoles requires a Rab GTPase, a large Rab effector complex, SNARE proteins which can form a 4-helical bundle, and the SNARE disassembly chaperones Sec17p and Sec18p. In addition to these proteins, specific vacuole lipids are required for efficient fusion in vivo and with the purified organelle. Reconstitution of vacuole fusion with all purified components reveals that high SNARE levels can mask the requirement for a complex mixture of vacuole lipids. At lower, more physiological SNARE levels, neutral lipids with small headgroups that tend to form non-bilayer structures (phosphatidylethanolamine, diacylglycerol, and ergosterol) are essential. Membranes without these three lipids can dock and complete trans-SNARE pairing but cannot rearrange their lipids for fusion. DOI: http://dx.doi.org/10.7554/eLife.01879.001.

KEYWORDS:

SNAREs; diacylglycerol; ergosterol; membrane fusion; phosphatidylethanolamine

PMID:
24596153
PMCID:
PMC3937803
DOI:
10.7554/eLife.01879
[Indexed for MEDLINE]
Free PMC Article

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