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PLoS One. 2014 Mar 3;9(3):e90780. doi: 10.1371/journal.pone.0090780. eCollection 2014.

Lycium barbarum polysaccharide prevents focal cerebral ischemic injury by inhibiting neuronal apoptosis in mice.

Author information

1
Department of Pharmacology, Ningxia Medical University, Yinchuan, People's Republic of China.
2
College of Nursing, Ningxia Medical University, Yinchuan, People's Republic of China.
3
Key Lab of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan, People's Republic of China.
4
Key Laboratory of Fertility Preservation and Maintenance, Ningxia Medical University, Yinchuan, People's Republic of China.
5
Department of Pharmacology, Ningxia Medical University, Yinchuan, People's Republic of China; Ningxia Hui Medicine Modern Engineering Research Center, Ningxia Medical University, Yinchuan, People's Republic of China.

Abstract

AIMS OF THE STUDY:

To investigate the neuroprotective effect of Lycium barbarum polysaccharide (LBP) on focal cerebral ischemic injury in mice and to explore its possible mechanism.

MATERIALS AND METHODS:

Male ICR mice were used to make the model of middle cerebral artery occlusion (MCAO) after intragastric administration with LBP (10, 20 and 40 mg/kg) and Nimodipine (0.4 mg/kg) for seven successive days. After 24 h of reperfusion, neurological scores were estimated and infarct volumes were measured by 2, 3, 5-triphenyltetrazolium chloride (TTC) staining. Morphological changes in ischemic brains were performed for hematoxylin-eosin (HE) staining. The number of apoptotic neurons was detected by TUNEL staining. The Bax, Bcl-2 protein expression and CytC, Caspase-3, -9 and cleaved PARP-1 activation were investigated by immunofluorescence and western-blot analysis.

RESULTS:

LBP (10, 20 and 40 mg/kg) treatment groups significantly reduced infract volume and neurological deficit scores. LBP also relieved neuronal morphological damage and attenuated the neuronal apoptosis. LBP at the dose of 40 mg/kg significantly suppressed overexpression of Bax, CytC, Caspase-3, -9 and cleaved PARP-1, and inhibited the reduction of Bcl-2 expression.

CONCLUSIONS:

Based on these findings we propose that LBP protects against focal cerebral ischemic injury by attenuating the mitochondrial apoptosis pathway.

PMID:
24595452
PMCID:
PMC3940937
DOI:
10.1371/journal.pone.0090780
[Indexed for MEDLINE]
Free PMC Article

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