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PLoS One. 2014 Mar 3;9(3):e90574. doi: 10.1371/journal.pone.0090574. eCollection 2014.

A unique mutation in a MYB gene cosegregates with the nectarine phenotype in peach.

Author information

1
Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Centro di Ricerca per la Frutticoltura (CRA-FRU), Rome, Italy.
2
Parco Tecnologico Padano, Via Einstein, Loc. C.na Codazza, Lodi, Italy.
3
Università di Bologna, DipSA, Bologna, Italy.
4
Università degli Studi di Milano, DiSAA, Milan, Italy.
5
IGA Technology Services, Udine, Italy.
6
Parco Tecnologico Padano, Via Einstein, Loc. C.na Codazza, Lodi, Italy; Università degli Studi di Milano, DiSAA, Milan, Italy.

Erratum in

  • PLoS One. 2014;9(10):e112032.

Abstract

Nectarines play a key role in peach industry; the fuzzless skin has implications for consumer acceptance. The peach/nectarine (G/g) trait was described as monogenic and previously mapped on chromosome 5. Here, the position of the G locus was delimited within a 1.1 cM interval (635 kb) based on linkage analysis of an F2 progeny from the cross 'Contender' (C, peach) x 'Ambra' (A, nectarine). Careful inspection of the genes annotated in the corresponding genomic sequence (Peach v1.0), coupled with variant discovery, led to the identification of MYB gene PpeMYB25 as a candidate for trichome formation on fruit skin. Analysis of genomic re-sequencing data from five peach/nectarine accessions pointed to the insertion of a LTR retroelement in exon 3 of the PpeMYB25 gene as the cause of the recessive glabrous phenotype. A functional marker (indelG) developed on the LTR insertion cosegregated with the trait in the CxA F2 progeny and was validated on a broad panel of genotypes, including all known putative donors of the nectarine trait. This marker was shown to efficiently discriminate between peach and nectarine plants, indicating that a unique mutational event gave rise to the nectarine trait and providing a useful diagnostic tool for early seedling selection in peach breeding programs.

PMID:
24595269
PMCID:
PMC3940905
DOI:
10.1371/journal.pone.0090574
[Indexed for MEDLINE]
Free PMC Article

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