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Org Lett. 2014 Mar 21;16(6):1676-9. doi: 10.1021/ol5003384. Epub 2014 Mar 5.

Rational domain swaps reveal insights about chain length control by ketosynthase domains in fungal nonreducing polyketide synthases.

Author information

1
Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, School of Pharmacy , Los Angeles, California 90089, United States.

Abstract

A facile genetic methodology in the filamentous fungus Aspergillus nidulans allowed exchange of the starter unit ACP transacylase (SAT) domain in the nonreduced polyketide synthase (NR-PKS) AfoE of the asperfuranone pathway with the SAT domains from 10 other NR-PKSs. The newly created hybrid with the NR-PKS AN3386 is able to accept a longer starter unit in place of the native substrate to create a novel aromatic polyketide in vivo.

PMID:
24593241
PMCID:
PMC3993715
DOI:
10.1021/ol5003384
[Indexed for MEDLINE]
Free PMC Article

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