Format

Send to

Choose Destination
Cold Spring Harb Protoc. 2014 Mar 1;2014(3):307-9. doi: 10.1101/pdb.prot073445.

In vivo delivery of lenti-Cre or adeno-Cre into mice using intranasal instillation.

Author information

1
Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Department of Pathology, Harvard Medical School, Charlestown, Massachusetts 02129;

Abstract

Lung cancer remains the leading cause of cancer deaths among both men and women, with a lower rate of survival than both breast and prostate cancer. Development of the Cre/lox system and improved mouse models have allowed researchers to gain a better understanding of human disease, including lung cancer. Through the viral delivery of Cre, gene function in adult mice can be precisely studied at a specific developmental stage or in a specific cell/tissue type of choice. This protocol describes how to produce adenovirus-Cre precipitate. Using this adeno-Cre (or lentivirus-Cre), Cre can be expressed in mouse lungs. The virus is delivered by intranasal instillation.

PMID:
24591689
PMCID:
PMC4169259
DOI:
10.1101/pdb.prot073445
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center